Anti-c-Kit (phospho Y703) antibody (ab62154)
Key features and details
- Rabbit polyclonal to c-Kit (phospho Y703)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Kit (phospho Y703) antibody
See all c-Kit primary antibodies -
Description
Rabbit polyclonal to c-Kit (phospho Y703) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human c-Kit around the phosphorylation site of tyrosine 703 (A-L-YP-K-N).
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Positive control
- WB: HepG2 treated with EGF cell lysate; Colon cancer lysate; U2OS lysate; Renal cancer cell line lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab62154 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Kit (phospho Y703) antibody (ab62154)All lanes : Anti-c-Kit (phospho Y703) antibody (ab62154) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) treated with EGF (200ng/ml, 30mins) cell lysate
Lane 2 : HepG2 treated with EGF (200ng/ml, 30mins) cell lysate with immunizing phosphopeptide
Predicted band size: 109 kDa
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Western blot - Anti-c-Kit (phospho Y703) antibody (ab62154) This image is courtesy of an Abreview submitted by Antibody SolutionsAll lanes : Anti-c-Kit (phospho Y703) antibody (ab62154)
Lane 1 : Colon cancer lysate (human)
Lane 2 : U2OS lysate (human)
Lane 3 : Renal cancer cell line (human)
Secondary
All lanes : Swine anti-rabbit HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 109 kDa
Exposure time: 6 minutes
