Anti-c-Kit antibody (ab178527)
Key features and details
- Goat polyclonal to c-Kit
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Kit antibody
See all c-Kit primary antibodies -
Description
Goat polyclonal to c-Kit -
Host species
Goat -
Specificity
ab178527 is expected to recognize both reported isoforms (NP_000213.1; NP_001087241.1). -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Goat, Cow, Cat, Dog, Pig, Common marmoset
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Immunogen
Synthetic peptide corresponding to Human c-Kit aa 685-697 (internal sequence). (NP_000213.1).
Sequence:KRDSFICSKQEDH
Database link: P10721 -
Positive control
- Human Hippocampus lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab178527 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Kit antibody (ab178527)All lanes : Anti-c-Kit antibody (ab178527) at 1 µg/ml
Lane 1 : Human Hippocampus lysate
Lane 2 : Human Hippocampus lysate with Blocking peptide added
Lysates/proteins at 35 µg per lane.
Predicted band size: 109 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-c-Kit antibody (ab178527)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab178527 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-c-Kit antibody (ab178527)Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation with ab178527 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
