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Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)

Key features and details

  • Mouse monoclonal [1G11] to EEA1 - Early Endosome Marker
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-EEA1 antibody [1G11] - Early Endosome Marker
    See all EEA1 primary antibodies

  • Description

    Mouse monoclonal [1G11] to EEA1 - Early Endosome Marker

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IFmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit

  • Immunogen

    Recombinant fragment corresponding to Human EEA1 aa 50 to the C-terminus.

  • Positive control

    • This anitbody gave a positive immunofluorescent signal in the following cell lines: HeLa HepG2

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
    Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
    ICC/IF image of ab70521 stained HepG2 cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70521 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.
  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
    Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
    ICC/IF image of ab70521 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70521, 10µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 oat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)
    Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521) This image is a courtesy of an anonymous abreview.

    ab70521 staining EEA1 in human THP-1 cells by Immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed, permeabilized in TritonX100 and blocked in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/200 (PBS + Tween20 + BSA 1%) and incubated with sample for 1 hour at 25°C. A 1.4 nm Gold (ultra-small) and Alexa Fluor® 594 conjugated goat polyclonal to mouse IgG1, diluted 1/500 was used as secondary. The red staining in image show EEA1 staining whilst green cytosolic staining corresponds to StarD5.

    See Abreview

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