Anti-EEA1 antibody - Early Endosome Marker (ab206860)
Key features and details
- Goat polyclonal to EEA1 - Early Endosome Marker
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EEA1 antibody - Early Endosome Marker
See all EEA1 primary antibodies -
Description
Goat polyclonal to EEA1 - Early Endosome Marker -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human EEA1 aa 800-900 (internal sequence). NP_003557.2.
Database link: Q15075 -
Positive control
- A549 and HEK293 lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab206860 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab206860)Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells permeabilized with 0.15% Triton staining EEA1. Primary incubation with ab206860 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.
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Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab206860)Anti-EEA1 antibody - Early Endosome Marker (ab206860) at 0.3 µg/ml + HEK293 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 162 kDa
