Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD83 antibody [EPR23809-19] (ab275021)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23809-19] to CD83
  • Suitable for: Flow Cyt, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD83 antibody [EPR23809-19]
    See all CD83 primary antibodies

  • Description

    Rabbit monoclonal [EPR23809-19] to CD83

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and Hodgkin's lymphoma tissue. Flow Cyt: HDLM-2 cells. IP: HDLM-2 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD83 antibody [EPR23809-19] (ab275021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD83 antibody [EPR23809-19] (ab275021)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD83 with ab275021 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab275021 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry - Anti-CD83 antibody [EPR23809-19] (ab275021)
    Flow Cytometry - Anti-CD83 antibody [EPR23809-19] (ab275021)

    Flow cytometric analysis of HDLM-2 (Human Hodgkin lymphoma) cells labelling CD83 with ab275021 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Immunoprecipitation - Anti-CD83 antibody [EPR23809-19] (ab275021)
    Immunoprecipitation - Anti-CD83 antibody [EPR23809-19] (ab275021)

    CD83 was immunoprecipitated from 0.35 mg HDLM-2 (human hodgkin lymphoma) whole cell lysate with ab275021 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275021 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HDLM-2 whole cell lysate 10 µg.

    Lane 2: ab275021 IP in HDLM-2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275021 in HDLM-2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 145 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 15320871).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD83 antibody [EPR23809-19] (ab275021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD83 antibody [EPR23809-19] (ab275021)

    Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CD83 with ab275021 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human Hodgkin's lymphoma (PMID: 24633193). The section was incubated with ab275021 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-CD83 antibody [EPR23809-19] (ab275021)
    Anti-CD83 antibody [EPR23809-19] (ab275021)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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