A-431 whole cell lysate (ab30132)
Overview
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Product name
A-431 whole cell lysate
See all A431 lysates -
Description
A-431 cell lysate -
General notes
Cell line: A431 (Human epithelial carcinoma).Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting. -
Storage buffer
Constituents: 1% 4-Nonylphenol, branched, ethoxylated, Potassium chloride, 0.1% Bromophenol blue, Magnesium chloride, 1.2% Beta mercaptoethanol, 2% Sodium lauryl sulfate, HEPES, Sucrose, 10% Glycerol
With Protease inhibitor cocktail/EDTA/Sodium deoxycholate -
Concentration information loading... -
Lysate notes
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. -
Research areas
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Background
A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.