Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Thursday March 04, 2021

Anti-CD40 antibody [EPR18005-4] (ab212058)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18005-4] to CD40
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse

Product name

Anti-CD40 antibody [EPR18005-4]
See all CD40 primary antibodies
Description

Rabbit monoclonal [EPR18005-4] to CD40
Host species

Rabbit
Specificity

ab212058 recognises a different epitope to ab188181.

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A20 whole cell lysate. ICC/IF: A20 cells. Flow Cyt: A20 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18005-4
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD40 antibody [EPR18005-4] (ab212058)

Flow cytometric analysis of A20 (mouse reticulum sarcoma cell line) cell line labeling CD40 with ab212058 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-4] (ab212058)

Immunofluorescent analysis of 100% methanol-fixed A20 (mouse reticulum sarcoma cell line) cells labeling CD40 with ab212058 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membranous staining on A20 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-CD40 antibody [EPR18005-4] (ab212058)

Anti-CD40 antibody [EPR18005-4] (ab212058) at 1/1000 dilution + A20 (mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 32 kDa
Observed band size: 42 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with the literature (PMID: 23404288 and PMID: 16709811).
Anti-CD40 antibody [EPR18005-4] (ab212058)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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