Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Thursday March 04, 2021

Anti-CD40 antibody [EPR18005-35] (ab188181)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18005-35] to CD40
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse

Product name

Anti-CD40 antibody [EPR18005-35]
See all CD40 primary antibodies
Description

Rabbit monoclonal [EPR18005-35] to CD40
Host species

Rabbit
Specificity

ab188181 recognises a different epitope to ab212058.

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A20 whole cell lysate. ICC/IF: A20 cells. Flow Cyt: A20 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18005-35
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD40 antibody [EPR18005-35] (ab188181)

Flow cytometric analysis of A20 (mouse reticulum sarcoma cell line) cell line labeling CD40 with ab188181 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-35] (ab188181)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 (mouse reticulum sarcoma cell line) cells labeling CD40 with ab188181 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on A20 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).
Western blot - Anti-CD40 antibody [EPR18005-35] (ab188181)

Anti-CD40 antibody [EPR18005-35] (ab188181) at 1/1000 dilution + A20 (mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 30 kDa
Observed band size: 32-42 kDa
why is the actual band size different from the predicted?

Blocking/Dilution: 5% NFDM/TBST.

Exposure: 15 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 23404288 and PMID: 16709811).
Anti-CD40 antibody [EPR18005-35] (ab188181)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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