Anti-CD300C antibody [EPR16470] (ab195811)
![Anti-CD300C antibody [EPR16470] (ab195811)](https://www.abcam.com/ps/products/195/ab195811/Images/ab195811-234914-anti-cd300c-antibody-epr16470-western-blot.jpg "Anti-CD300C antibody [EPR16470] (ab195811)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16470] to CD300C
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CD300C antibody [EPR16470]
See all CD300C primary antibodies -
Description
Rabbit monoclonal [EPR16470] to CD300C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- THP1, Human fetal spleen and fetal brain, Mouse heart, kidney and spleen, Rat heart and spleen, RAW 264.7, PC12 and NIH 3T3 lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16470 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD300C antibody [EPR16470] (ab195811)All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat spleen lysate
Lane 6 : RAW 264.7 lysate
Lane 7 : PC12 lysate
Lane 8 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 25 kDaThe observed MW is consistent with what has been described in the literature (PMID:20656921)
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![Western blot - Anti-CD300C antibody [EPR16470] (ab195811)](https://www.abcam.com/ps/products/195/ab195811/Images/ab195811-234912-anti-cd300c-antibody-epr16470-western-blot.jpg)
Western blot - Anti-CD300C antibody [EPR16470] (ab195811)All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 25 kDaThe observed MW is consistent with what has been described in the literature (PMID:20656921)
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![Western blot - Anti-CD300C antibody [EPR16470] (ab195811)](https://www.abcam.com/ps/products/195/ab195811/Images/ab195811-234911-anti-cd300c-antibody-epr16470-western-blot.jpg)
Western blot - Anti-CD300C antibody [EPR16470] (ab195811)Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution + THP1 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 25 kDaThe observed MW is consistent with what has been described in the literature (PMID:20656921)
-
![Anti-CD300C antibody [EPR16470] (ab195811)](https://www.abcam.com/ps/products/195/ab195811/Images/ab195811-5-benefits-of-recombinant-antibodies.png)
Anti-CD300C antibody [EPR16470] (ab195811)
