Anti-PI 3 Kinase p55 gamma antibody (ab235234)
Key features and details
- Goat polyclonal to PI 3 Kinase p55 gamma
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PI 3 Kinase p55 gamma antibody
See all PI 3 Kinase p55 gamma primary antibodies -
Description
Goat polyclonal to PI 3 Kinase p55 gamma -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: PI 3 Kinase p55 gamma overexpressing HEK-293T whole cell lysate; mouse testis lysate. IHC-P: Human tonsil tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PI 3 Kinase p55 gamma antibody (ab235234)Anti-PI 3 Kinase p55 gamma antibody (ab235234) at 1/1000 dilution + Mouse testis lysate
Secondary
Goat IgG-HRP at 1/5000 dilution
Predicted band size: 54 kDa
Exposure time: 1 second
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Western blot - Anti-PI 3 Kinase p55 gamma antibody (ab235234)All lanes : Anti-PI 3 Kinase p55 gamma antibody (ab235234) at 1/1000 dilution
Lane 1 : PI 3 Kinase P55 gamma overexpressing HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Empty vector HEK-293T lysate
Secondary
All lanes : Goat IgG HRP at 1/40000 dilution
Predicted band size: 54 kDa
Exposure time: 75 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p55 gamma antibody (ab235234)Paraffin-embedded human tonsil tissue stained for PI 3 Kinase p55 gamma using ab235234 at 1/100 dilution for 30 minutes at room temperature, in immunohistochemical analysis. Secondary antibody was Donkey anti-Goat (HRP) (Ready to use). Counterstained with hematoxylin.
HIER using Citrate Buffer for 20min.
Panel A) Human testis H&E stain.
Panel B) ab235234 staining at 20X.
Panel C) ab235234 staining at 40X. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p55 gamma antibody (ab235234)Paraffin-embedded human tonsil tissue stained for PI 3 Kinase p55 gamma using ab235234 at 1/100 dilution for 30 minutes at room temperature, in immunohistochemical analysis. Secondary antibody was Donkey anti-Goat (HRP) 4 µg/ml for 20 minutes at room temperature. Counterstained with hematoxylin.
HIER using Citrate Buffer for 20min.
Panel A) Human tonsil H&E stain.
Panel B) ab235234 staining at 20X.
Panel C) ab235234 staining at 40X. -
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p55 gamma antibody (ab235234)100% methanol-fixed, 0.3% Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PI 3 Kinase p55 gamma (Green) using ab235234 at 15 µg/ml overnight at 4°C. The secondary antibody was Donkey anti-Goat IgG DiLight™ 488 Conjugated antibody at 15 µg/ml for 1 hour at room temperature. The nuclear counterstain was DAPI (Blue).
(A) DAPI
(B) ab235234 + Secondary
(C) Merge A+B
(D) Secondary only
Expected localization: Cytosol (UniProt), Nucleoplams (HPA) – distinct punctate staining in nucleus could indicate nucleoplasm.
