Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10376] to NR1D1 + RVR - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NR1D1 antibody [EPR10376] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR10376] to NR1D1 + RVR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human uterus tissue. Human colon carcinoma tissue. Flow Cyt (Intra): HeLa cells. ICC/IF: HeLa cells.

  • General notes

    ab236008 is the carrier-free version of ab174309.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)
    Flow Cytometry - Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NR1D1 with ab174309 at 1/20 dilution(10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174309).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling NR1D1 with ab174309 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174309).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)
    Immunocytochemistry/ Immunofluorescence - Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)

    Immunofluorescent analysis of HeLa cells labeling NR1D1 with ab174309 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174309).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)

    Immunohistochemical analysis of paraffin-embedded human uterus tissue labeling NR1D1 with ab174309 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174309).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)
    Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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