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Signal Transduction Growth Factors/Hormones Hormones

Anti-NR1D1 antibody [EPR10376] (ab174309)

Price and availability

328 339 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-NR1D1 antibody [EPR10376] (ab174309)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10376] to NR1D1
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-NR1D1 antibody [EPR10376]
    See all NR1D1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10376] to NR1D1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NR1D1 aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P20393

  • Positive control

    • Y79, Hela, HepG2 and Jurkat cell lysates; Colon carcinoma tissue; Uterus tissue; Hela cells
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10376
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Thyroid axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Orphan Nuclear Receptors

Images

  • Western blot - Anti-NR1D1 antibody [EPR10376] (ab174309)
    Western blot - Anti-NR1D1 antibody [EPR10376] (ab174309)
    All lanes : Anti-NR1D1 antibody [EPR10376] (ab174309) at 1/1000 dilution

    Lane 1 : Y79 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 67 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 antibody [EPR10376] (ab174309)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 antibody [EPR10376] (ab174309)

    Immunohistochemical analysis of paraffin-embedded human uterus tissue labeling NR1D1 with ab174309 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NR1D1 antibody [EPR10376] (ab174309)
    Immunocytochemistry/ Immunofluorescence - Anti-NR1D1 antibody [EPR10376] (ab174309)

    Immunofluorescent analysis of HeLa cells labeling NR1D1 with ab174309 at 1/100 dilution.

  • Flow Cytometry - Anti-NR1D1 antibody [EPR10376] (ab174309)
    Flow Cytometry - Anti-NR1D1 antibody [EPR10376] (ab174309)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NR1D1 with ab174309 at 1/20 dilution(10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 antibody [EPR10376] (ab174309)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 antibody [EPR10376] (ab174309)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling NR1D1 with ab174309 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-NR1D1 antibody [EPR10376] (ab174309)
    Anti-NR1D1 antibody [EPR10376] (ab174309)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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