Anti-SMCHD1 antibody (ab31865)
Key features and details
- Rabbit polyclonal to SMCHD1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMCHD1 antibody
See all SMCHD1 primary antibodies -
Description
Rabbit polyclonal to SMCHD1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide corresponding to Human SMCHD1 aa 1750-1850 conjugated to keyhole limpet haemocyanin.
(Peptide available asab31864)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanAll applications MouseApplication Abreviews Notes ICC/IF (1) 1/100.Notes ICC/IF
1/100.Target
- Information by UniProt
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Database links
- Entrez Gene: 23347 Human
- Entrez Gene: 74355 Mouse
- Omim: 614982 Human
- SwissProt: A6NHR9 Human
- SwissProt: Q6P5D8 Mouse
- Unigene: 8118 Human
- Unigene: 194450 Mouse
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Alternative names
- BAMS antibody
- FSHD2 antibody
- KIAA0650 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)This image is courtesy of an Abreview submitted by Dr Kirk McManusab31865 (1/100) staining SMCHD1 by immunofluorescence in assynchronous HeLa cells (green). Secondary antibody: goat polyclonal antibody conjugated to Cy3; Counterstain: DAPI (red). Please refer to Abreview for further information.
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Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)ICC/IF image of ab31865 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31865, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methaol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
Protocols
Datasheets and documents
References (14)
ab31865 has been referenced in 14 publications.
- Dion C et al. SMCHD1 is involved in de novo methylation of the DUX4-encoding D4Z4 macrosatellite. Nucleic Acids Res N/A:N/A (2019). PubMed: 30698748
- Balog J et al. Monosomy 18p is a risk factor for facioscapulohumeral dystrophy. J Med Genet 55:469-478 (2018). PubMed: 29563141
- de Greef JC et al. Smchd1 haploinsufficiency exacerbates the phenotype of a transgenic FSHD1 mouse model. Hum Mol Genet 27:716-731 (2018). PubMed: 29281018
- Mason AG et al. SMCHD1 regulates a limited set of gene clusters on autosomal chromosomes. Skelet Muscle 7:12 (2017). PubMed: 28593035
- Mason AG et al. SMCHD1 regulates a limited set of gene clusters on autosomal chromosomes. Skelet Muscle 7:12 (2017). ChIP ; Human . PubMed: 28587678
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865) This image is courtesy of an Abreview submitted by Dr Kirk McManusab31865 (1/100) staining SMCHD1 by immunofluorescence in assynchronous HeLa cells (green). Secondary antibody: goat polyclonal antibody conjugated to Cy3; Counterstain: DAPI (red). Please refer to Abreview for further information.
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Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)ICC/IF image of ab31865 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31865, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methaol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
