Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Friday March 12, 2021

Anti-ALKBH4 antibody [EPR19216] (ab195379)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19216] to ALKBH4
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-ALKBH4 antibody [EPR19216]
See all ALKBH4 primary antibodies
Description

Rabbit monoclonal [EPR19216] to ALKBH4
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver, heart and kidney tissue lysate; human pancreas tissue lysate; HEK-293, MCF7, MOLT-4 and WI-38 whole cell lysate. IP: HEK-293 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19216
Isotype

IgG
Research areas

Western blot - Anti-ALKBH4 antibody [EPR19216] (ab195379)

All lanes : Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution

Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 33 kDa
Observed band size: 33 kDa

Exposure time: 1 minute

Blocking/Dilution: 5% NFDM/TBST.
Western blot - Anti-ALKBH4 antibody [EPR19216] (ab195379)

All lanes : Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 4 : Human pancreas tissue lysate
Lane 5 : WI-38 (human fetal lung fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 33 kDa
Observed band size: 33 kDa

Exposure time: 1 minute

Blocking/Dilution: 5% NFDM/TBST.
Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] (ab195379)

ALKBH4 was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab195379 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab195379 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293 whole cell lysate 10ug (Input).
Lane 2: ab195379 IP in HEK-293 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195379 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
Anti-ALKBH4 antibody [EPR19216] (ab195379)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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