Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALKBH4 antibody [EPR19216] (ab195379)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19216] to ALKBH4
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ALKBH4 antibody [EPR19216]
    See all ALKBH4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19216] to ALKBH4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, heart and kidney tissue lysate; human pancreas tissue lysate; HEK-293, MCF7, MOLT-4 and WI-38 whole cell lysate. IP: HEK-293 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19216

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ALKBH4 antibody [EPR19216] (ab195379)
    Western blot - Anti-ALKBH4 antibody [EPR19216] (ab195379)
    All lanes : Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 1 minute


    Blocking/Dilution: 5% NFDM/TBST.

  • Western blot - Anti-ALKBH4 antibody [EPR19216] (ab195379)
    Western blot - Anti-ALKBH4 antibody [EPR19216] (ab195379)
    All lanes : Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 4 : Human pancreas tissue lysate
    Lane 5 : WI-38 (human fetal lung fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 1 minute


    Blocking/Dilution: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] (ab195379)
    Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] (ab195379)

    ALKBH4 was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab195379 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab195379 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: HEK-293 whole cell lysate 10ug (Input).
    Lane 2: ab195379 IP in HEK-293 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195379 in HEK-293 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds.

  • Anti-ALKBH4 antibody [EPR19216] (ab195379)
    Anti-ALKBH4 antibody [EPR19216] (ab195379)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ALKBH4 antibody [EPR19216] (ab195379)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.