Anti-NOLC1 antibody [EPR14896] (ab184550)
![Anti-NOLC1 antibody [EPR14896] (ab184550)](https://www.abcam.com/ps/products/184/ab184550/Images/ab184550-217184-anti-nolc1-antibody-epr14896-immunofluorescence.jpg "Anti-NOLC1 antibody [EPR14896] (ab184550)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14896] to NOLC1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-NOLC1 antibody [EPR14896]
See all NOLC1 primary antibodies -
Description
Rabbit monoclonal [EPR14896] to NOLC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562 and HeLa cell lysates, Human prostate tissue, K562 and HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14896 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NOLC1 antibody [EPR14896] (ab184550)
Immunofluorescence staining of -20℃ Acetone fixed K562 cells labeling NOLC1 using ab184550 at a 1/500 dilution (Red). Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution. Counterstained with Dapi (blue).
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![Flow Cytometry - Anti-NOLC1 antibody [EPR14896] (ab184550)](https://www.abcam.com/ps/products/184/ab184550/Images/ab184550-217185-anti-nolc1-antibody-epr14896-flow-cytometry.jpg)
Flow Cytometry - Anti-NOLC1 antibody [EPR14896] (ab184550)Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling NOLC1 with ab184550 at a 1/300 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLC1 antibody [EPR14896] (ab184550)](https://www.abcam.com/ps/products/184/ab184550/Images/ab184550-217183-anti-nolc1-antibody-epr14896-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLC1 antibody [EPR14896] (ab184550)Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling NOLC1 at a 1/100 dilution, counterstained with Hematoxylin.
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![Western blot - Anti-NOLC1 antibody [EPR14896] (ab184550)](https://www.abcam.com/ps/products/184/ab184550/Images/ab184550-217182-anti-nolc1-antibody-epr14896-western-blot.jpg)
Western blot - Anti-NOLC1 antibody [EPR14896] (ab184550)All lanes : Anti-NOLC1 antibody [EPR14896] (ab184550) at 1/50000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 74 kDa
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![Anti-NOLC1 antibody [EPR14896] (ab184550)](https://www.abcam.com/ps/products/184/ab184550/Images/ab184550-5-benefits-of-recombinant-antibodies.png)
Anti-NOLC1 antibody [EPR14896] (ab184550)
