Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NOLC1 antibody [EPR14896] (ab184550)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14896] to NOLC1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-NOLC1 antibody [EPR14896]
    See all NOLC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14896] to NOLC1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562 and HeLa cell lysates, Human prostate tissue, K562 and HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NOLC1 antibody [EPR14896] (ab184550)
    Immunocytochemistry/ Immunofluorescence - Anti-NOLC1 antibody [EPR14896] (ab184550)

    Immunofluorescence staining of -20℃ Acetone fixed K562 cells labeling NOLC1 using ab184550 at a 1/500 dilution (Red). Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution. Counterstained with Dapi (blue).

  • Flow Cytometry - Anti-NOLC1 antibody [EPR14896] (ab184550)
    Flow Cytometry - Anti-NOLC1 antibody [EPR14896] (ab184550)

    Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling NOLC1 with ab184550 at a 1/300 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLC1 antibody [EPR14896] (ab184550)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOLC1 antibody [EPR14896] (ab184550)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling NOLC1 at a 1/100 dilution, counterstained with Hematoxylin.

  • Western blot - Anti-NOLC1 antibody [EPR14896] (ab184550)
    Western blot - Anti-NOLC1 antibody [EPR14896] (ab184550)
    All lanes : Anti-NOLC1 antibody [EPR14896] (ab184550) at 1/50000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 74 kDa

  • Anti-NOLC1 antibody [EPR14896] (ab184550)
    Anti-NOLC1 antibody [EPR14896] (ab184550)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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