Antibodies, Proteins, Kits and Reagents for Life Science

Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free (ab242382)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1974Y] to MCM7/PRL - BSA and Azide free
Suitable for: ICC/IF, IHC-P, IP, WB, Flow Cyt
Reacts with: Rat, Human

Product name

Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free
See all MCM7/PRL primary antibodies
Description

Rabbit monoclonal [EP1974Y] to MCM7/PRL - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, IHC-P, IP, WB, Flow Cytmore details
Species reactivity

Reacts with: Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, A431 and C6 cell lysates; Rat spleen tissue lysate. IHC-P: Human tonsil and rat stomach tissue. IP: Jurkat cell lysate. Flow Cyt: MCF7 cells. ICC/IF: MCF7 cells
General notes
ab242382 is the carrier-free version of ab52489 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab242382 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as MCM7
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1974Y
Isotype

IgG
Research areas

Western blot - Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free (ab242382)

All lanes : Anti-MCM7/PRL antibody [EP1974Y] (ab52489) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 4 : Rat spleen lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 81 kDa
Observed band size: 81 kDa

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52489).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free (ab242382)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling MCM7/PRL with Purified ab52489 at 1/50 dilution (5.4 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52489).
Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free (ab242382)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MCM7/PRL with Purified ab52489 at 1/50 (5.4 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52489).
Immunoprecipitation - Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free (ab242382)

Purified ab52489 at 1/20 dilution (2ug) immunoprecipitating MCM7/PRL in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate (10µg)
Lane 2 (+): ab52489 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52489 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 81 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52489).
Flow Cytometry - Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free (ab242382)

Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MCM7/PRL with Purified ab52489 at 1/30 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52489).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free (ab242382)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling MCM7/PRL with Purified ab52489 at 1/50 dilution (5.4 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52489).
Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free (ab242382)

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