Anti-MCM7/PRL antibody (ab96849)
Key features and details
- Rabbit polyclonal to MCM7/PRL
- Suitable for: ChIP, WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCM7/PRL antibody
See all MCM7/PRL primary antibodies -
Description
Rabbit polyclonal to MCM7/PRL -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish
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Immunogen
Recombinant fragment corresponding to Human MCM7/PRL aa 14-275.
Database link: P33993 -
Positive control
- WB: H1299 cell lysate. IHC-P: Cal27 Xenograft tissue. ICC/IF: HeLa cells. IP: HeLa cells. ChIP: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM7/PRL antibody (ab96849)Anti-MCM7/PRL antibody (ab96849) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 81 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody (ab96849)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using ab96849 at 1/200 dilution (1).Merged with DNA probe (2).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody (ab96849)Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft using ab96849 at 1/100 dilution.
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Immunoprecipitation - Anti-MCM7/PRL antibody (ab96849)MCM7 was immunoprecipitated from 40 μg of HeLa whole cell lysate with ab96849 at 1/1000 dilution. EasyBlot anti-rabbit IgG, was used as secondary antibody.
Lane 1: 40 μg HeLa whole cell extract (Input).
Lane 2: Control with 4 μg of preimmune Rabbit IgG.
Lane 3: Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal.7.5 % SDS-PAGE
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ChIP - Anti-MCM7/PRL antibody (ab96849)Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
