Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17671] to AKT1 + AKT2 + AKT3
Suitable for: Flow Cyt, IP, WB, IHC-P
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671]
See all AKT1 + AKT2 + AKT3 primary antibodies
Description

Rabbit monoclonal [EPR17671] to AKT1 + AKT2 + AKT3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: AKT3 recombinant protein fragment (His-Tag®): aa351-479; AKT2 recombinant protein fragment (His-Tag®): aa282-481; AKT1 recombinant protein fragment (His-Tag®): aa281-480; A549 whole cell lysate; Human fetal brain and fetal kidney lysates; Mouse brain lysate; Rat brain and heart lysates. IHC-P: Human cerebral cortex, Human adenocarcinoma of colon, mouse cerebral cortex and rat kidney tissues. ICC/IF: HeLa cells. Flow Cytometry: A549 cells. IP: A549 whole cell extract.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17671
Isotype

IgG
Research areas

Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/5000 dilution

Lane 1 : AKT3 recombinant protein fragment (His-Tag®): aa351-479
Lane 2 : AKT2 recombinant protein fragment (His-Tag®): aa282-481
Lane 3 : AKT1 recombinant protein fragment (His-Tag®): aa281-480

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
The bottom panel is probed with an anti-His tag antibody.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/2000 dilution + A549 (Human lung carcinoma) whole cell lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/2000 dilution

Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal kidney lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633) at 1/2000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lane 3 : Rat heart lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on neurons of the Human cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on Human adenocarcinoma of colon is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on neurons of the mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and weak cytoplasmic staining on rat kidney is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

AKT1 + AKT2 + AKT3 was immunoprecipitated from 1mg of A549 (Human lung carcinoma) whole cell extract with ab185633 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab185633 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1: A549 whole cell extract 10 µg (Input). Lane 2: ab185633 IP in A549 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185633 in A549 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Flow Cytometry - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

Flow cytometric analysis of 2% paraformaldehyde-fixed A549 (Human lung carcinoma) cells labeling AKT1 + AKT2 + AKT3 with ab185633 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Anti-AKT1 + AKT2 + AKT3 antibody [EPR17671] (ab185633)

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