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Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody
    See all AKT1 + AKT2 + AKT3 primary antibodies
  • Description

    Rabbit polyclonal to AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316)
  • Host species

    Rabbit
  • Specificity

    ab131443 detects endogenous levels of AKT1/2/3 only when phosphorylated at tyrosine 315/316/312.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human AKT1 + AKT2 + AKT3 conjugated to keyhole limpet haemocyanin.
    Sequence:

    P-E-Y(p)-L-A)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HepG2 cell extract treated with EGF; HeLa cells; Human breast carcinoma tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131443 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKB / AKT
    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • AKT

Images

  • Western blot - Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)
    Western blot - Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)
    All lanes : Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443) at 1/500 dilution

    Lane 1 : HepG2 cell extract untreated
    Lane 2 : HepG2 cell extract treated with EGF

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)
    Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)

    Immunofluorescent analysis of methanol-fixed HeLa cells labelling AKT1 + AKT2 + AKT3 (phospho Y315 + Y316 + Y312) with ab131443 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling AKT1 + AKT2 + AKT3 (phospho Y315 + Y316 + Y312) with ab131443 at 1/50 dilution. Right panel was preincubated with blocking peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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