Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR16798] to AKT1 + AKT2 + AKT3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798]
    See all AKT1 + AKT2 + AKT3 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR16798] to AKT1 + AKT2 + AKT3

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human AKT1 + AKT2 + AKT3 aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Other SwissProt IDs: P31751, Q9Y243
    Database link: P31749

  • Positive control

    • WB: Human fetal kidney, mouse brain and rat brain tissue lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)
    Western blot - HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)
    All lanes : HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854) at 1/5000 dilution

    Lane 1 : Kidney (Human) Tissue Lysate - fetal normal tissue
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200854 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)
    HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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