HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)
![HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)](https://www.abcam.com/ps/products/200/ab200854/Images/ab200854-252098-WBab2008541.jpg "HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR16798] to AKT1 + AKT2 + AKT3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798]
See all AKT1 + AKT2 + AKT3 primary antibodies -
Description
HRP Rabbit monoclonal [EPR16798] to AKT1 + AKT2 + AKT3 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
-
Immunogen
Recombinant fragment within Human AKT1 + AKT2 + AKT3 aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Other SwissProt IDs: P31751, Q9Y243
Database link: P31749 -
Positive control
- WB: Human fetal kidney, mouse brain and rat brain tissue lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16798 -
Isotype
IgG -
Research areas
Images
-
Western blot - HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)All lanes : HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854) at 1/5000 dilution
Lane 1 : Kidney (Human) Tissue Lysate - fetal normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200854 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)](https://www.abcam.com/ps/products/200/ab200854/Images/ab200854-3-benefits-of-recombinant-antibodies.png)
HRP Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab200854)
