Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Thursday March 04, 2021

Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17737] to AKT1 + AKT2 + AKT3
Suitable for: WB, ICC/IF
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737]
See all AKT1 + AKT2 + AKT3 primary antibodies
Description

Rabbit monoclonal [EPR17737] to AKT1 + AKT2 + AKT3
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: AKT1, AKT2 and AKT3 recombinant protein fragments; MCF7, HepG2, PC-3, Jurkat, 293, C6 and PC-12 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain, mouse spleen, rat brain and rat heart lysates. ICC/IF: MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17737
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AKT1 + AKT2 + AKT3 with ab200195 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Cytoplasm staining on MCF7 cell line is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab200195 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)

All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution

Lane 1 : AKT3 recombinant protein fragment (His-Tag®): aa351-479
Lane 2 : AKT2 recombinant protein fragment (His-Tag®): aa282-481
Lane 3 : AKT1 recombinant protein fragment (His-Tag®): aa281-480

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa

Exposure time: 3 seconds

AKT3 recombinant protein fragment contains aa351-479 with His-Tag®. AKT2 recombinant protein fragment contains aa282-481 with His-Tag® and AKT1 recombinant protein fragment contains aa281-480 with His-Tag®.
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)

Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)

Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)

All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/10000 dilution

Lane 1 : PC-3 (Human prostate cancer cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : 293 (Human embryonic kidney) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)

All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)

All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)

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