Anti-AKT1 + AKT2 + AKT3 antibody (ab82538)
Key features and details
- Rabbit polyclonal to AKT1 + AKT2 + AKT3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AKT1 + AKT2 + AKT3 antibody
See all AKT1 + AKT2 + AKT3 primary antibodies -
Description
Rabbit polyclonal to AKT1 + AKT2 + AKT3 -
Host species
Rabbit -
Specificity
ab82538 recognizes AKT1, AKT2, and AKT3. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human AKT1 + AKT2 + AKT3 aa 471-475.
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Positive control
- Human recombinant AKT1, AKT2, and AKT3; HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab82538)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab82538) at 1/1000 dilution
Lane 1 : human recombinant AKT1
Lane 2 : human recombinant AKT2
Lane 3 : human recombinant AKT3
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 56 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
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Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab82538)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab82538) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK-293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : THP1 cell lysate
Lane 5 : 3T6 cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
