Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD24 antibody [ML5] - BSA and Azide free (ab278510)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [ML5] to CD24 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD24 antibody [ML5] - BSA and Azide free
    See all CD24 primary antibodies

  • Description

    Mouse monoclonal [ML5] to CD24 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: MCF7 cells. Flow Cyt: MCF7 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab278510 is the carrier-free version of ab278509. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278510 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Images

  • Immunocytochemistry - Anti-CD24 antibody [ML5] - BSA and Azide free (ab278510)
    Immunocytochemistry - Anti-CD24 antibody [ML5] - BSA and Azide free (ab278510)

    This data was developed using ab278509 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 cells labelling CD24 with ab278509 at 1/50 (18.44 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab278509 at a 1/50 dilution followed by ab150080 at a 1/200 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

    Confocal image showing membranous staining in MCF7 cell line.

    Negative control: MDA-MB-231 (PMID: 18433506). 

  • Flow Cytometry - Anti-CD24 antibody [ML5] - BSA and Azide free (ab278510)
    Flow Cytometry - Anti-CD24 antibody [ML5] - BSA and Azide free (ab278510)

    This data was developed using ab278509 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of MDA-MB-231 (Human breast adenocarcinoma epithelial cells) (Left panel) and MCF7 (Human breast adenocarcinoma epithelial cells) (Right panel) cells labelling CD24 with ab278509 at 1/1000 dilution (0.1µg) (Red) compared with a isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    Negative control: MDA-MB-231 (PMID: 18433506).

    Gated on viable cells.

  • Anti-CD24 antibody [ML5] - BSA and Azide free (ab278510)
    Anti-CD24 antibody [ML5] - BSA and Azide free (ab278510)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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