Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µl 274 732 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7899] to HDGF
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-HDGF antibody [EPR7899]
See all HDGF primary antibodies
Description

Rabbit monoclonal [EPR7899] to HDGF
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to residues at the C terminus of Human HDGF (UniProt P51858).
Positive control
- A549, Saos-2, ECV-304, and 293T cell lysates; A549 cells; Human colon tissue
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7899
Isotype

IgG
Research areas

Western blot - Anti-HDGF antibody [EPR7899] (ab131046)

All lanes : Anti-HDGF antibody [EPR7899] (ab131046) at 1/10000 dilution

Lane 1 : A549 cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : ECV-304 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 27 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDGF antibody [EPR7899] (ab131046)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HDGF with ab131046 antibody at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-HDGF antibody [EPR7899] (ab131046)

Immunofluorescence analysis of A549 cells labelling HDGF with ab131046 antibody at 1/250 dilution.
Anti-HDGF antibody [EPR7899] (ab131046)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com