Anti-HDGF antibody [26C4a] (ab58644)
![Anti-HDGF antibody [26C4a] (ab58644)](https://www.abcam.com/ps/products/58/ab58644/Images/ab58644.gif "Anti-HDGF antibody [26C4a] (ab58644)")
Key features and details
- Mouse monoclonal [26C4a] to HDGF
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-HDGF antibody [26C4a]
See all HDGF primary antibodies -
Description
Mouse monoclonal [26C4a] to HDGF -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment of human HDGF, from an internal sequence.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab58644 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
26C4a -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-HDGF antibody [26C4a] (ab58644)Anti-HDGF antibody [26C4a] (ab58644) at 1/100 dilution + immunising recombinant protein
at 25 µg
Secondary
Sheep Anti Mouse IgG at 1/3000 dilution
Predicted band size: 27 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The immunogen shown in the WB is tagged.
