Anti-CD62L antibody [EPR17012-27] (ab253240)
![Anti-CD62L antibody [EPR17012-27] (ab253240)](https://www.abcam.com/ps/products/253/ab253240/Images/ab253240-2-253240FC1.jpg "Anti-CD62L antibody [EPR17012-27] (ab253240)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17012-27] to CD62L
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CD62L antibody [EPR17012-27]
See all CD62L primary antibodies -
Description
Rabbit monoclonal [EPR17012-27] to CD62L -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse serum. Flow Cyt: Mouse splenocytes.
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General notes
ab253240 (EPR17012-27) is the capture of the ssELISA kit ab199085.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17012-27 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (ab253240)
Flow cytometric analysis of mouse splenocytes labeling CD62L with ab253240 at 1/60 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or anti-CD62L RabMab (Right). Then stained with anti-CD4 conjugated with Alexa Fluor® 647 and anti-CD44 conjugated with BV421. While CD4+ splenocytes express CD62L and CD44, their expressions are mutually exclusive.
Gated on viable CD4+ T cells.
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![Western blot - Anti-CD62L antibody [EPR17012-27] (ab253240)](https://www.abcam.com/ps/products/253/ab253240/Images/ab253240-1-253240WB1.jpg)
Western blot - Anti-CD62L antibody [EPR17012-27] (ab253240)All lanes : Anti-CD62L antibody [EPR17012-27] (ab253240) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse lymph node lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 75-100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 12165530).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-CD62L antibody [EPR17012-27] (ab253240)](https://www.abcam.com/ps/products/253/ab253240/Images/ab253240-3-benefits-of-recombinant-antibodies.png)
Anti-CD62L antibody [EPR17012-27] (ab253240)
