Anti-Histone H4 (acetyl K12) antibody (ab61238)
Key features and details
- Rabbit polyclonal to Histone H4 (acetyl K12)
- Suitable for: IHC-P, WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (acetyl K12) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (acetyl K12) -
Host species
Rabbit -
Specificity
ab61238 detects endogenous levels of Histone H4 only when acetylated at lysine 12. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Histone H4 aa 10-15 (acetyl K12). Synthetic acetylated peptide derived from human Histone H4 around the acetylation site of lysine 12
LGK(Ac)GG
Sequence:LGK(Ac)GG
Database link: P62805 -
Positive control
- WB: Extracts from COS7 cells, treated with TSA (400nM, 24hours); KB cells, HEK-293 cells (with TSA 400nM 24h). IHC-P: Human breast carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H4 (acetyl K12) antibody (ab61238) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of acetylated peptide.
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All lanes : Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution
Lane 1 : Extracts from COS7 cells, treated
with TSA (400nM, 24hours) with no acetylated peptide
Lane 2 : Extracts from COS7 cells, treated
with TSA (400nM, 24hours) with acetylated peptide
Predicted band size: 11 kDa
Observed band size: 11 kDa
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Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution + KB cells
Predicted band size: 11 kDa
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All lanes : Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution
Lane 1 : HEK-293 cells (with TSA 400nM 24h)
Lane 2 : HEK-293 cells (without TSA 400nM 24h)
Predicted band size: 11 kDa