Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3 (acetyl K56) antibody [RM179] (ab238307)

Key features and details

Rabbit monoclonal [RM179] to Histone H3 (acetyl K56)
Suitable for: ELISA, WB, ICC/IF
Reacts with: Human, Recombinant fragment
Isotype: IgG

Product name

Anti-Histone H3 (acetyl K56) antibody [RM179]
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [RM179] to Histone H3 (acetyl K56)
Host species

Rabbit
Specificity

ab238307 reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with other acetylated Lysines in histone H3.
Tested applications

Suitable for: ELISA, WB, ICC/IFmore details
Species reactivity

Reacts with: Human, Recombinant fragment
Immunogen

Synthetic peptide corresponding to Human Histone H3 (acetyl K56).
Database link: P84243
Positive control
- WB: Acid extracts of HeLa cells treated with sodium butyrate. ICC/IF: HeLa cells treated with sodium butyrate. HepG2 cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from an animal origin–free culture supernatant.
Clonality

Monoclonal
Clone number

RM179
Isotype

IgG
Research areas

Western blot - Anti-Histone H3 (acetyl K56) antibody [RM179] (ab238307)

All lanes : Anti-Histone H3 (acetyl K56) antibody [RM179] (ab238307) at 1 µg/ml

Lane 1 : Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma cells treated with sodium butyrate
Lane 2 : Recombinant Histone H3.3

Predicted band size: 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K56) antibody [RM179] (ab238307)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H3 (acetyl K56) using ab238307 (red) at 2 µg/ml in ICC/IF.

Actin filaments have been labeled with fluorescein phalloidin (green).
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K56) antibody [RM179] (ab238307)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Histone H3 (acetyl K56) using ab238307 at 2 µg/ml in ICC/IF.
ELISA - Anti-Histone H3 (acetyl K56) antibody [RM179] (ab238307)

ab238307 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac).

No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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