Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (acetyl K4) antibody [EPR16596] - BSA and Azide free (ab240195)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16596] to Histone H3 (acetyl K4) - BSA and Azide free
  • Suitable for: WB, Dot blot, ICC/IF, ChIP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Histone H3 (acetyl K4) antibody [EPR16596] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16596] to Histone H3 (acetyl K4) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Dot blot, ICC/IF, ChIPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240195 is the carrier-free version of ab176799. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240195 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16596

  • Isotype

    IgG

  • Research areas

Images

  • ChIP - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - BSA and Azide free (ab240195)
    ChIP - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - BSA and Azide free (ab240195)

    Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab176799 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176799).

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - BSA and Azide free (ab240195)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - BSA and Azide free (ab240195)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, treated with Trichostatin A (500 ng/ml) for 4 hours or untreated, labeling Histone H3 (acetyl K4) with ab176799 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line. The expression increased after treatment with Trichostatin A (500 ng/ml) for 4 hours.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab176799 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176799).

  • Dot Blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - BSA and Azide free (ab240195)
    Dot Blot - Anti-Histone H3 (acetyl K4) antibody [EPR16596] - BSA and Azide free (ab240195)

    Dot blot analysis of Histone H3 (acetyl K4) labeled with ab176799 at 1/1000 dilution.

    Lane 1: H3 (acetyl K4) peptide

    Lane 2: H3 (formylK4) peptide

    Lane 3: H3 (methylK4) peptide

    Lane 4: H3 (crotonylK4) peptide

    Lane 5: Unmodified H3 peptide

    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated was used as secondary antobody at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176799).

  • Anti-Histone H3 (acetyl K4) antibody [EPR16596] - BSA and Azide free (ab240195)
    Anti-Histone H3 (acetyl K4) antibody [EPR16596] - BSA and Azide free (ab240195)

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