Anti-VPS15 antibody [EPR5302(2)] (ab128903)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5302(2)] to VPS15
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-VPS15 antibody [EPR5302(2)]
See all VPS15 primary antibodies -
Description
Rabbit monoclonal [EPR5302(2)] to VPS15 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Synthetic peptide within Human VPS15 aa 50-150. The exact sequence is proprietary.
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Positive control
- WB: Raji, C6, and Neuro-2a whole cell lysates. Mouse 14.5 brain, Rat E14 brain, and Rat P0 brain lysates.
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General notes
This product was previously labelled as PI 3 Kinase regulatory subunit 4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.22 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5302(2) -
Isotype
IgG -
Research areas
Images
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Anti-VPS15 antibody [EPR5302(2)] (ab128903) at 1/1000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This antibody detects high background.
Blocking/Diluting buffer: 5% NFDM/TBST
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All lanes : Anti-VPS15 antibody [EPR5302(2)] (ab128903) at 1/500 dilution (Purified)
Lane 1 : Mouse E14.5 brain lysates
Lane 2 : Rat E14 brain lysates
Lane 3 : Rat P0 brain lysates
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST
This antibody detects high background.
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