Anti-VPS15 antibody (ab240914)
Key features and details
- Rabbit polyclonal to VPS15
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-VPS15 antibody
See all VPS15 primary antibodies -
Description
Rabbit polyclonal to VPS15 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Orangutan
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Immunogen
Synthetic peptide corresponding to Human VPS15 aa 1300 to the C-terminus (C terminal).
Database link: Q99570 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VPS15 antibody (ab240914)
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling VPS15 (green) using ab240914 at 1/100 dilution in ICC/IF, followed by a goat anti-rabbit ATTO 488 conjugated secondary antibody at 1/200 dilution. Both primary and secondary antibodies were incubated for 1 hour at RT. Counterstained with Phalloidin Texas Red F-Actin stain (red) at 1/1000 for 1 hour at RT. The nuclear counterstain in DAPI (blue) at 1/5000 for 5 minutes at RT.
(A) DAPI (blue) nuclear stain
(B) Phalloidin Texas Red F-Actin stain (red)
(C) ab240914
(D) Composite
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Western blot - Anti-VPS15 antibody (ab240914)Anti-VPS15 antibody (ab240914) at 1/1000 dilution + Rat brain lysate at 15 µg
Secondary
HRP-linked goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 153 kDa
Exposure time: 6 minutesBlocked with 5% skim milk in 1XTBST.
Primary and secondary incubation: 1 hour at RT.
