Anti-VPS15 antibody - C-terminal (ab229665)
Key features and details
- Rabbit polyclonal to VPS15 - C-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VPS15 antibody - C-terminal
See all VPS15 primary antibodies -
Description
Rabbit polyclonal to VPS15 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment within Human VPS15 (C terminal). The exact sequence is proprietary.
Database link: Q99570 -
Positive control
- WB: Jurkat, Raji, NCI-H929, HEK-293T, A431, HeLa, HepG2, Neuro2A, C8D30, NIH-3T3, Raw264.7 and C2C12 whole cell extracts; Mouse liver tissue extract. IP: HeLa whole cell extract. IHC-P: Mouse duodenum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VPS15 antibody - C-terminal (ab229665)All lanes : Anti-VPS15 antibody - C-terminal (ab229665) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : PIK3R4 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 153 kDa5% SDS-PAGE gel.
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Western blot - Anti-VPS15 antibody - C-terminal (ab229665)All lanes : Anti-VPS15 antibody - C-terminal (ab229665) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 153 kDa5% SDS-PAGE gel.
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Western blot - Anti-VPS15 antibody - C-terminal (ab229665)All lanes : Anti-VPS15 antibody - C-terminal (ab229665) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 153 kDa5% SDS-PAGE gel.
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Western blot - Anti-VPS15 antibody - C-terminal (ab229665)All lanes : Anti-VPS15 antibody - C-terminal (ab229665) at 1/1000 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell extract
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 5 : C2C12 (Mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 153 kDa5% SDS-PAGE gel.
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Western blot - Anti-VPS15 antibody - C-terminal (ab229665)Anti-VPS15 antibody - C-terminal (ab229665) at 1/1000 dilution + Mouse liver tissue extract at 50 µg
Predicted band size: 153 kDa5% SDS-PAGE gel.
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Immunoprecipitation - Anti-VPS15 antibody - C-terminal (ab229665)VPS15 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg of ab229665. Western blot was performed from the immunoprecipitate using ab229665. Anti-rabbit IgG was used as the secondary antibody.
Lane 1: HeLa whole cell extract (input).
Lane 2: Control IgG instead of ab229665 in HeLa whole cell extract.
Lane 3: ab229665 IP in HeLa whole cell extract. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS15 antibody - C-terminal (ab229665)Paraffin-embedded mouse duodenum tissue stained for VPS15 using ab229665 at 1/500 dilution in immunohistochemical analysis.
