Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFS6 antibody [EPR15957] (ab195807)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15957] to NDUFS6
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NDUFS6 antibody [EPR15957]
    See all NDUFS6 primary antibodies

  • Description

    Rabbit monoclonal [EPR15957] to NDUFS6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal heart, Human skeletal muscle, Mouse brain, Mouse heart, Rat brain, Rat heart and Rat kidney lysates; Human tonsil, Human hepatocellular carcinoma and Rat liver tissues; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Western blot - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Anti-NDUFS6 antibody [EPR15957] (ab195807) at 1/10000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 13 kDa

  • Western blot - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Western blot - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Anti-NDUFS6 antibody [EPR15957] (ab195807) at 1/10000 dilution + Human fetal heart at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 13 kDa

  • Western blot - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Western blot - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Anti-NDUFS6 antibody [EPR15957] (ab195807) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 13 kDa

  • Western blot - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Western blot - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    All lanes : Anti-NDUFS6 antibody [EPR15957] (ab195807) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate
    Lane 5 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 13 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] (ab195807)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NDUFS6 with ab195807 at 1/500. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Inset image: negative control obtained using PBS instead of ab195807 and secondary antibody only.
    Note: Cytoplasm staining on human tonsil tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] (ab195807)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling NDUFS6 with ab195807 at 1/500. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    Inset image: negative control obtained using PBS instead of ab195807 and secondary antibody only.
    Note: Cytoplasm staining on human hepatocellular carcinoma tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] (ab195807)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling NDUFS6 with ab195807 at 1/500. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    Inset image: negative control obtained using PBS instead of ab195807 and secondary antibody only.
    Note: Cytoplasm staining on rat liver tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Immunoprecipitation - Anti-NDUFS6 antibody [EPR15957] (ab195807)

    Immunoprecipitation analysis of Human fetal kidney lysate labeling NDUFS6 using ab195807 at 1/30 dilution (Lane 2).

    Lane 3: IP using Rabbit monoclonal IgG (ab172730) ) instead of ab195807 in Human fetal kidney lysates.

    Lane 1: Input: 10 μg Human fetal kidney lysates.

    Subsequent WB detection was performed using ab195807 at 1/1000 dilution.

    An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

  • Anti-NDUFS6 antibody [EPR15957] (ab195807)
    Anti-NDUFS6 antibody [EPR15957] (ab195807)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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