Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15957-37] to NDUFS6
Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-NDUFS6 antibody [EPR15957-37]
See all NDUFS6 primary antibodies
Description

Rabbit monoclonal [EPR15957-37] to NDUFS6
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal kidney, fetal brain, fetal heart , skeletal muscle; Mouse brain, heart and kidney; Rat brain and PC12 lysates; Human cervix squamous cell carcinoma and kidney tissues; HeLa and NIH 3T3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15957-37
Isotype

IgG
Research areas

Flow Cytometry - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

Flow cytometric analysis of NIH 3T3 cells (paraformaldehyde-fixed, 2%) labeling NDUFS6 with ab195808 at 1/90 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

Immunofluorescent analysis of HeLa cells (100% methanol-fixed, 0.1% tritonX-100 permeabilized) labeling NDUFS6 with ab195808 at 1/200 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).
Note: Cytoplasm staining on HeLa cell line was observed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling NDUFS6 with ab195808 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

Note: Cytoplasm staining on human squamous cell carcinoma of cervix tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFS6 with ab195808 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

Note: Cytoplasm staining on human kidney tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/1000 dilution + PC12 cell lysate

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 14 kDa

Exposure time: 3 minutes
Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 14 kDa

Exposure time: 10 seconds
Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/10000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human skeletal muscle lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 14 kDa

Exposure time: 30 seconds
Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/10000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 14 kDa

Exposure time: 3 minutes
Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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