Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15957-37] to NDUFS6
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NDUFS6 antibody [EPR15957-37]
    See all NDUFS6 primary antibodies

  • Description

    Rabbit monoclonal [EPR15957-37] to NDUFS6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney, fetal brain, fetal heart , skeletal muscle; Mouse brain, heart and kidney; Rat brain and PC12 lysates; Human cervix squamous cell carcinoma and kidney tissues; HeLa and NIH 3T3 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    Flow Cytometry - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

    Flow cytometric analysis of NIH 3T3 cells (paraformaldehyde-fixed, 2%) labeling NDUFS6 with ab195808 at 1/90 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    Immunocytochemistry/ Immunofluorescence - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

    Immunofluorescent analysis of  HeLa cells (100% methanol-fixed, 0.1% tritonX-100 permeabilized) labeling NDUFS6 with ab195808 at 1/200 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution  and counter-stained with DAPI (blue).

    Note: Cytoplasm staining on HeLa cell line was observed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

    Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling NDUFS6 with ab195808 at 1/500 dilution  followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cytoplasm staining on human squamous cell carcinoma of cervix tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFS6 with ab195808 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cytoplasm staining on human kidney tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/1000 dilution + PC12 cell lysate

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa


    Exposure time: 3 minutes
  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa


    Exposure time: 10 seconds
  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/10000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human skeletal muscle lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa


    Exposure time: 30 seconds
  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    Western blot - Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa


    Exposure time: 3 minutes
  • Anti-NDUFS6 antibody [EPR15957-37] (ab195808)
    Anti-NDUFS6 antibody [EPR15957-37] (ab195808)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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