Anti-ITPKA antibody (ab251867)
Key features and details
- Rabbit polyclonal to ITPKA
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ITPKA antibody
See all ITPKA primary antibodies -
Description
Rabbit polyclonal to ITPKA -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human ITPKA aa 300-400.
Database link: P23677 -
Positive control
- IHC-P: Human cerebral cortex, glioma and caudate tissues; Mouse brain, hypothalamus and subiculum tissues. ICC/IF: U-251 MG cells. WB: Human cerebral cortex tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for ITPKA (green) using ab251867 at 4 µg/ml in ICC/IF.
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Anti-ITPKA antibody (ab251867) at 0.04 µg/ml + Human cerebral cortex tissue lysate
Predicted band size: 51 kDaWestern blot analysis of human cerebral cortex tissue lysate labeling ITPKA with ab251867 at 0.04 ug/ml.
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Formalin-fixed, paraffin-embedded mouse hypothalamus tissue stained for ITPKA with ab251867 at a 1/2500 dilution in immunohistochemical analysis.
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Paraffin-embedded human cerebral cortex tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse subiculum tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.
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Paraffin-embedded human glioma tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.
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Paraffin-embedded human caudate tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.
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Paraffin-embedded human liver tissue shows absence of immunoreactivity for ITPKA when incubated with ab251867 at 1/2500 dilution (negative control).
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Paraffin-embedded mouse brain tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.