Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-IGFBP6 antibody [EPR21809] (ab219560)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21809] to IGFBP6
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-IGFBP6 antibody [EPR21809]
See all IGFBP6 primary antibodies
Description

Rabbit monoclonal [EPR21809] to IGFBP6
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human IGFBP6 recombinant protein; Human placenta and kidney lysates; NCI-H1299 and HaCaT whole cell lysates. ICC/IF: HaCaT cells. Flow Cyt: HaCaT cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21809
Isotype

IgG
Research areas

Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)

All lanes : Anti-IGFBP6 antibody [EPR21809] (ab219560) at 1/1000 dilution

Lane 1 : Human placenta lysate
Lane 2 : Human kidney lysate
Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 4 : HaCaT (human keratinocyte cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 25 kDa
Observed band size: 34 kDa
why is the actual band size different from the predicted?

Exposure time: 59 seconds

Blocking/dilution buffer and concentration: 5% NFDM/TBST.

The molecular mass observed is consistent with the literature (PMID 27623076; PMID 21820463).
Immunocytochemistry/ Immunofluorescence - Anti-IGFBP6 antibody [EPR21809] (ab219560)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells labeling IGFBP6 with ab219560 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HaCaT cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)

Anti-IGFBP6 antibody [EPR21809] (ab219560) at 1/5000 dilution + Human IGFBP6 recombinant protein (aa25-240) from mammalian expression system at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 25 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Flow Cytometry - Anti-IGFBP6 antibody [EPR21809] (ab219560)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HaCaT (human keratinocyte cell line) cell line labeling IGFBP6 with ab219560 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-IGFBP6 antibody [EPR21809] (ab219560)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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