Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IGFBP6 antibody [EPR21809] (ab219560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21809] to IGFBP6
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-IGFBP6 antibody [EPR21809]
    See all IGFBP6 primary antibodies

  • Description

    Rabbit monoclonal [EPR21809] to IGFBP6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human IGFBP6 recombinant protein; Human placenta and kidney lysates; NCI-H1299 and HaCaT whole cell lysates. ICC/IF: HaCaT cells. Flow Cyt: HaCaT cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
    Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
    All lanes : Anti-IGFBP6 antibody [EPR21809] (ab219560) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : Human kidney lysate
    Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 4 : HaCaT (human keratinocyte cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 25 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?


    Exposure time: 59 seconds


    Blocking/dilution buffer and concentration: 5% NFDM/TBST.

    The molecular mass observed is consistent with the literature (PMID 27623076; PMID 21820463).

  • Immunocytochemistry/ Immunofluorescence - Anti-IGFBP6 antibody [EPR21809] (ab219560)
    Immunocytochemistry/ Immunofluorescence - Anti-IGFBP6 antibody [EPR21809] (ab219560)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells labeling IGFBP6 with ab219560 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HaCaT cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
    Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
    Anti-IGFBP6 antibody [EPR21809] (ab219560) at 1/5000 dilution + Human IGFBP6 recombinant protein (aa25-240) from mammalian expression system at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 25 kDa
    Observed band size: 34 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

  • Flow Cytometry - Anti-IGFBP6 antibody [EPR21809] (ab219560)
    Flow Cytometry - Anti-IGFBP6 antibody [EPR21809] (ab219560)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HaCaT (human keratinocyte cell line) cell line labeling IGFBP6 with ab219560 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-IGFBP6 antibody [EPR21809] (ab219560)
    Anti-IGFBP6 antibody [EPR21809] (ab219560)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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