Helicobacter pylori IgA plus ELISA Kit (ab178644)
Key features and details
- Range: 1.14 IU/ml - 150 IU/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Helicobacter pylori IgA plus ELISA Kit
See all Helicobacter pylori IgA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% sample 24 1.007% 3.69% sample 24 1.574% 2.43% sample 24 0.635% 3.59% Inter-assay Sample n Mean SD CV% sample 12 7.34% 5.02% sample 12 5.09% 11.49% sample 12 21.04% 4.33% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Range
1.14 IU/ml - 150 IU/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Helicobacter pylori IgA plus ELISA Kit (ab178644) is designed for the quantitative determination of IgA class antibodies against Helicobacter pylori in human serum or plasma (citrate, heparin).
The quantitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
Helicobacter pylori is a spiral Gram-negative bacterium (2-6.5 μm in size, flagellated) which colonizes the human gastric mucosa. The organism is found in the mucus layer and adheres to the surface mucus epithelium of the stomach but generally does not penetrate the gastric mucosa directly.
However, there is a secondary inflammatory response in the mucosa leading to chronic active gastritis. Helicobacter pylori is the primary causative agent in most cases of peptic ulcer disease. Infection rate in Europe is about 30%-40%, worldwide about 50%. There is an inverse relationship between the presence of Helicobacter pylori infection and socioeconomic status. In developing countries, people acquire the infection at an early age such that by young adulthood as many as 90% of the population might have Helicobacter pylori gastritis. In developed western countries the prevalence of Helicobacter pylori gastritis is much lower. Under these conditions, the rate of acquisition is much slower (roughly 1% per annum) and the older one is, the more likely one is to be infected with the organism.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml anti-human IgA HRP conjugate 1 x 20ml Cover Foil 1 unit Helicobacter pylori Coated Microplate (IgA) 1 unit Helicobacter pylori IgA Additional Control 1 x 2ml Helicobacter pylori IgA Standard A - 0 U/mL 1 x 2ml Helicobacter pylori IgA Standard B - 15 U/mL 1 x 2ml Helicobacter pylori IgA Standard C - 50 U/mL 1 x 2ml Helicobacter pylori IgA Standard D - 150 U/mL 1 x 2ml IgA Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Typical Standard Curve – Representative standard curve using ab178644 using the point to point function.