Anti-SP3 antibody - N-terminal (ab227856)
Key features and details
- Rabbit polyclonal to SP3 - N-terminal
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SP3 antibody - N-terminal
See all SP3 primary antibodies -
Description
Rabbit polyclonal to SP3 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human SP3 (N terminal). The exact sequence is proprietary.
Database link: Q02447 -
Positive control
- WB: HeLa cell extracts; Jurkat whole cell and nuclear extracts; Raji and NCI-H929 whole cell extracts. IP: Jurkat nuclear extract. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma and ovarian carcinoma tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SP3 antibody - N-terminal (ab227856)All lanes : Anti-SP3 antibody - N-terminal (ab227856) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : SP3 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 82 kDa
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Western blot - Anti-SP3 antibody - N-terminal (ab227856)All lanes : Anti-SP3 antibody - N-terminal (ab227856) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : SP3 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 82 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-SP3 antibody - N-terminal (ab227856)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SP3 (green) using ab227856 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 5 minutes.
Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-SP3 antibody - N-terminal (ab227856)SP3 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 µg ab227856. Western blot was performed from the immunoprecipitate using ab227856. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Jurkat whole cell extract.
Lane 2: Control IgG IP in Jurkat whole cell extract.
Lane 3: ab227856 IP in Jurkat whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP3 antibody - N-terminal (ab227856)Paraffin-embedded human breast carcinoma tissue stained for SP3 using ab227856 at 1/1000 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP3 antibody - N-terminal (ab227856)Paraffin-embedded human ovarian carcinoma tissue stained for SP3 using ab227856 at 1/1000 dilution in immunohistochemical analysis.
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Western blot - Anti-SP3 antibody - N-terminal (ab227856)All lanes : Anti-SP3 antibody - N-terminal (ab227856) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 82 kDa7.5 % SDS-PAGE gel.
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Western blot - Anti-SP3 antibody - N-terminal (ab227856)All lanes : Anti-SP3 antibody - N-terminal (ab227856) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 82 kDa7.5 % SDS-PAGE gel.
