Immunohistochemical analysis of formalin fixed, paraffin-embedded human tonsil staining HLA-DR with ab257320 at 2µg/ml for 30 minutes at room temperature.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257320).

Immunocytochemistry/ Immunofluorescent analysis of Ramos cells staining HLA-DR with ab257320 at 2µg/ml, followed by a goat anti-rabbit IgG-CF488 secondary (blue) and an isotype control (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257320).

Flow Cytometric analysis of Raji cells staining HLA-DR with ab257320, followed by a goat anti-rabbit IgG-CF488 secondary antiobdy (blue). Isotype control (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257320).

Immunocytochemistry/ Immunofluorescent analysis of Ramos cells staining HLA-DR with ab257320 at 2µg/ml, followed by a goat anti-rabbit IgG-CF488 secondary (green). Nuceli stained with reddot (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257320).

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.