Anti-HLA-DR antibody [DA6.147] - BSA and Azide free (ab244584)
![Anti-HLA-DR antibody [DA6.147] - BSA and Azide free (ab244584)](https://www.abcam.com/ps/products/244/ab244584/Images/ab244584-366354-AntiHLADR-antibody-DA6147-BSA-Azide-free-western-blot-raji-ramos-human.jpg "Anti-HLA-DR antibody [DA6.147] - BSA and Azide free (ab244584)")
Key features and details
- Mouse monoclonal [DA6.147] to HLA-DR - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HLA-DR antibody [DA6.147] - BSA and Azide free
See all HLA-DR primary antibodies -
Description
Mouse monoclonal [DA6.147] to HLA-DR - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human breast and tonsil tissue. WB: Raji and Ramos whole cell lysate.
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General notes
ab244584 is the PBS only version of ab238469.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
DA6.147 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-HLA-DR antibody [DA6.147] - BSA and Azide free (ab244584)All lanes : Anti-HLA-DR antibody [DA6.147] (ab238469) at 1 µg/ml
Lane 1 : Raji whole cell lysate
Lane 2 : Ramos whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 29 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab238469).
This blot was produced using a 4-12% Bis-tris under the MES buffer system. The gel was run at 200V for m5 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab238469 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [DA6.147] - BSA and Azide free (ab244584)](https://www.abcam.com/ps/products/244/ab244584/Images/ab244584-366355-AntiHLADR-antibody-DA6147-BSA-Azide-free-immunohistochemistry-breast-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [DA6.147] - BSA and Azide free (ab244584)This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab238469).
IHC image of HLA-DR staining in a section of formalin-fixed paraffin-embedded normal human breast* performed on a Leica BONDab238469, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [DA6.147] - BSA and Azide free (ab244584)](https://www.abcam.com/ps/products/244/ab244584/Images/ab244584-366356-AntiHLADR-antibody-DA6147-BSA-Azide-free-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [DA6.147] - BSA and Azide free (ab244584)This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab238469).
IHC image of HLA-DR staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDab238469, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
