Anti-iNOS antibody [K13-A] (ab136918)
Key features and details
- Rabbit monoclonal [K13-A] to iNOS
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-iNOS antibody [K13-A]
See all iNOS primary antibodies -
Description
Rabbit monoclonal [K13-A] to iNOS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide corresponding to a region of Human iNOS.
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Epitope
Antibody recognizes the epitope located between Ser1118 - Gly1129 -
Positive control
- Mouse brain tissue lysate - total protein (ab30151) can be used as a positive control in WB. Raw264.7 whole cell lysate (LPS treated)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 0.32% Tris HCl, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability. -
Clonality
Monoclonal -
Clone number
K13-A -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution
Lane 1 : Raw264.7 Whole Cell Lysate
Lane 2 : Raw264.7 Whole Cell Lysate (LPS stimulated)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anit Rabbit IR680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 36 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands.This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Mouse IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx
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Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution + iNOS Overexpression Lysate (Human) at 0.1 µg
Secondary
Goat anti Rabbit IR680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx
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Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 131 kDa