Anti-DDAH1 antibody [EPR13921] - BSA and Azide free (ab250530)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13921] to DDAH1 - BSA and Azide free
- Suitable for: IHC-P, IP, WB, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-DDAH1 antibody [EPR13921] - BSA and Azide free
See all DDAH1 primary antibodies -
Description
Rabbit monoclonal [EPR13921] to DDAH1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HUVEC, A431 and HepG2 cell lysates
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General notes
Ab250530 is the carrier-free version of ab181859. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250530 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13921 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-DDAH1 antibody [EPR13921] (ab181859) at 1/10000 dilution
Lane 1 : wild-type A431 cell lysate
Lane 2 : DDAH1 knockout A431 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab181859).
Lanes 1 - 4: Merged signal (red and green). Green - ab181859 observed at 37 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab181859 was shown to react with DDAH1 in A431 wild-type cells in Western blot. Loss of signal was observed when DDAH1 knockout sample was used. A431 wild-type and DDAH1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab181859 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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