Recombinant human KDM2B protein (ab196435)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human KDM2B protein
See all KDM2B proteins and peptides -
Biological activity
Enzyme was incubated with 0.4 μM substrate in HEPES-containing JMJ assay buffer SA and SA- (without KG) in a 10-μl reaction for 180 min at 30°C. 5 μl of Anti-Rabbit acceptor beads (1:250) and 5 μl of rabbit polyclonal antibody (1:200) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 μl) and mixture was further incubated for 10 min at RT before counts were measured.
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Purity
70 - 90% by HPLC. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAGPQMGGSAEDHPPRKRHAAEKQKKKTVIYTKCFEFESATQRPIDRQRY DENEDLSDVEEIVSVRGFSLEEKLRSQLYQGDFVHAMEGKDFNYEYVQRE ALRVPLIFREKDGLGIKMPDPDFTVRDVKLLVGSRRLVDVMDVNTQKGTE MSMSQFVRYYETPEAQRDKLYNVISLEFSHTKLEHLVKRPTVVDLVDWVD NMWPQHLKEKQTEATNAIAEMKYPKVKKYCLMSVKGCFTDFHIDFGGTSV WYHVFRGGKIFWLIPPTLHNLALYEEWVLSGKQSDIFLGDRVERCQRIEL KQGYTFFIPSGWIHAVYTPVDSLVFGGNILHSFNVPMQLRIYEIEDRTRV QPKFRYPFYYEMCWYVLERYVYCVTQRSHLTQEYQRESMLIDAPRKPSID GFSSDSWLEMEEEACDQQPQEEEEKDEEGEGRDRAPKPPTDGSTSPTSTP SEDQEALGKKPKAPALRFLKRTLSNESEESVKSTTLAVDYPKTPTGSPAT EVSAKWTHLTEFELKGLKALVEKLESLPENKKCVPEGIEDPQALLEGVKN VLKEHADDDPSLAITGVPVVTWPKKTPKNRAVGRPKGKLGPASAVKLAAN RTTAGARRRRTRCRKCEACLRTECGECHFCKDMKKFGGPGRMKQSCIMRQ -
Predicted molecular weight
75 kDa including tags -
Amino acids
1 to 650 -
Tags
DDDDK tag C-Terminus -
Additional sequence information
GenBank Accession No.: NM_032590
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human KDM2B protein (ab196435)
SDS-PAGE analysis of 2.4 μg of ab196435 on a 4-20% SDS gel stained with Coomassie.
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SDS-PAGE - Recombinant human KDM2B protein (ab196435)SDS-PAGE analysis of 2.8 μg of ab196435 on a 4-20% SDS gel stained with Coomassie.
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Functional Studies - Recombinant human KDM2B protein (ab196435)Specific activity of ab196435
