Recombinant human PHF8 protein (Active) (ab271712)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PHF8 protein (Active)
See all PHF8 proteins and peptides -
Biological activity
Specific Activity: 0.064 pmol/min/μg
Assay conditions: PHF8 enzyme is incubated with biotinylated histone H3 peptide substrate in HEPES-based buffer in a 10 µg reaction for 1 hr. at RT. Add 5 µg of AlphaLISA™ anti-Rabbit Acceptor beads (20 µg/ml), followed by addition of 5 µl of antibody and 30 min. incubation at RT. Add 10 µg of AlphaScreen™ Streptavidin-conjugated donor beads (40 µg/ml). Alpha counts are measured after 10 min. RT incubation.
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Purity
>= 95 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
NRSRAIVQR GRVLPPPAPL DTTNLAGRRT LQGRAKMASV PVYCLCRLPY DVTRFMIECD MCQDWFHGSC VGVEEEKAAD IDLYHCPNCE VLHGPSIMKK RRGSSKGHDT HKGKPVKTGS PTFVRELRSR TFDSSDEVIL KPTGNQLTVE FLEENSFSVP ILVLKKDGLG MTLPSPSFTV RDVEHYVGSD KEIDVIDVTR QADCKMKLGD FVKYYYSGKR EKVLNVISLE FSDTRLSNLV ETPKIVRKLS WVENLWPEEC VFERPNVQKY CLMSVRDSYT DFHIDFGGTS VWYHVLKGEK IFYLIRPTNA NLTLFECWSS SSNQNEMFFG DQVDKCYKCS VKQGQTLFIP TGWIHAVLTP VDCLAFGGNF LHSLNIEMQL KAYEIEKRLS TADLFRFPNF ETICWYVGKH ILDIFRGLRE NRRHPASYLV HGGKALNLAF RAWTRKEALP DHEDEIPETV RTVQLIKDLA REIRLVEDIF QQNVGKTSNI FGLQRIFPAG SIPLTRPAHS TSVSMSRLSL PSKNG -
Predicted molecular weight
62 kDa including tags -
Amino acids
2 to 525 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
- Histone lysine demethylase PHF8
- JHDM1F
- Jumonji C domain containing histone demethylase 1F
Images
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Functional Studies - Recombinant human PHF8 protein (Active) (ab271712)
Specific activity of ab271712 was 0.064 pmol/min/μg.
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SDS-PAGE - Recombinant human PHF8 protein (Active) (ab271712)SDS-PAGE analysis of 3 µg ab271712.