Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1288Y] to CD18 (phospho S745)
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CD18 (phospho S745) antibody [EP1288Y]
    See all CD18 primary antibodies

  • Description

    Rabbit monoclonal [EP1288Y] to CD18 (phospho S745)

  • Host species

    Rabbit

  • Specificity

    This antibody only detects CD18 phosphorylated on Serine 745.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CD18 (phospho S745). The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat, NIH/3T3 and PC-12 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Raw 246.7

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
    Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
    All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 0.9 µg/ml (purified)

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates 15ug. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa



    Blocking and diluting buffer: 5% NFDM/TBST
  • Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
    Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
    All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 0.9 µg/ml (purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa



    Blocking and diluting buffer: 5% NFDM/TBST
  • Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
    Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
    All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 0.9 µg/ml (purified)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa



    Blocking and diluting buffer: 5% NFDM/TBST
  • Immunoprecipitation - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
    Immunoprecipitation - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)

    ab52920 (purified) at 1:50 dilution (2µg) immunoprecipitating CD18 in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab52920 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52920 in Jurkat whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
    Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
    All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 1/500 dilution (unpurified)

    Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : AP-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa

  • Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
    Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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