Anti-NQO1 antibody [EPR21230] (ab213239)
![Anti-NQO1 antibody [EPR21230] (ab213239)](https://www.abcam.com/ps/products/213/ab213239/Images/ab213239-314132-anti-nqo1-antibody-epr21230-western-blot.jpg "Anti-NQO1 antibody [EPR21230] (ab213239)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21230] to NQO1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-NQO1 antibody [EPR21230]
See all NQO1 primary antibodies -
Description
Rabbit monoclonal [EPR21230] to NQO1 -
Host species
Rabbit -
Specificity
Based on internal western blot testing, this antibody showed no reactivity to NQO1 in mouse samples. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Recombinant fragment.
Database link: P15559 -
Positive control
- WB: HAP1, HepG2, Human Kidney
-
General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR21230 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-NQO1 antibody [EPR21230] (ab213239)All lanes : Anti-NQO1 antibody [EPR21230] (ab213239) at 1 µg/ml
Lane 1 : HAP1 wild-type whole cell lysate at 40 µg
Lane 2 : NQO1 knockout whole cell lysate at 40 µg
Lane 3 : HepG2 whole cell lysate at 20 µg
Lane 4 : MEF1 whole cell lysate at 20 µg
Lane 5 : Human Kidney tissue lysate at 20 µg
Lane 6 : Mouse Kidney tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 31 kDaab213239 was shown to specifically react with NQO1 in wild type HAP1 cells. No band was observed when NQO1 knockout samples were used. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. The membrane was then blocked for an hour using 3% milk before being incubated with ab213239 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at a concentration of 1ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Anti-NQO1 antibody [EPR21230] (ab213239)](https://www.abcam.com/ps/products/213/ab213239/Images/ab213239-2-benefits-of-recombinant-antibodies.png)
Anti-NQO1 antibody [EPR21230] (ab213239)
