Anti-NNMT antibody (ab272669)
Key features and details
- Rabbit polyclonal to NNMT
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NNMT antibody
See all NNMT primary antibodies -
Description
Rabbit polyclonal to NNMT -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NNMT aa 150-300.
Database link: P40261 -
Positive control
- WB: U-2 OS cells. ICC: SiHa cells. IHC-P: Human liver, kidney and skin tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NNMT antibody (ab272669)
PFA fixed, Triton X-100 permeablized SiHa cells (human cervical carcinoma cell line) labeling NNMT (green) using ab272669 at 2 ug/ml in ICC/IF analysis.
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Western blot - Anti-NNMT antibody (ab272669)All lanes : Anti-NNMT antibody (ab272669) at 0.4 µg/ml
Lane 1 : U-2 OS cells (Human bone osteosarcoma epithelial cell line)
Lane 2 : SK-MEL-30 cells (Human cutaneous melanoma cell line)Corresponding NNMT RNA-seq data are presented for the same cell lines.
Loading control: Anti-GAPDH.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody (ab272669)Immunohistochemical analysis of human liver tissue labeling NNMT with ab272669 at a 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody (ab272669)Immunohistochemical analysis of human kidney tissue labeling NNMT with ab272669 at a 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody (ab272669)Immunohistochemical analysis of human pancreas tissue labeling NNMT with ab272669 at a 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody (ab272669)Immunohistochemical analysis of human skin tissue labeling NNMT with ab272669 at a 1/500 dilution.
