Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)

Key features and details

  • Mouse monoclonal [A180] to NQO1 - BSA and Azide free
  • Suitable for: ICC, Sandwich ELISA, WB, ELISA, Flow Cyt, IP, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-NQO1 antibody [A180] - BSA and Azide free
    See all NQO1 primary antibodies

  • Description

    Mouse monoclonal [A180] to NQO1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, Sandwich ELISA, WB, ELISA, Flow Cyt, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant full length protein corresponding to Human NQO1.

  • Positive control

    • WB: HAP1 and HepG2 whole cell lysates; human kidney tissue lysate. ICC: HepG2 cells. Flow cyt: HeLa cells. IHC-P: FFPE human breast adenocarcinoma and pancreas adenocarcinoma tissue sections.

  • General notes

    ab264434 is the carrier-free version of ab28947.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)
    Western blot - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: NQO1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab28947 observed at 31 kDa. Red - loading control, ab176560, observed at 50 kDa.

    ab28947 was shown to specifically react with NQO1 in wild-type HAP1 cells as signal was lost in NQO1 knockout cells. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. ab28947 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and Azide (ab28947).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)

    IHC image of NQO1 staining in sections of formalin fixed paraffin embedded normal human pancreas* (left) and human pancreas adenocarcinoma* (right), performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28947, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and Azide (ab28947).

  • Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)
    Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)

    ICC/IF image of ab28947 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab28947, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and Azide (ab28947).

  • Flow Cytometry - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)
    Flow Cytometry - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)

    Overlay histogram showing HeLa cells stained with ab28947 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28947, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and Azide (ab28947).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)

    IHC image of NQO1 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28947, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and Azide (ab28947).

  • Sandwich ELISA - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)
    Sandwich ELISA - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)

    Sandwich ELISA for the detection of NQO1, using ab28947 (1/500) as the capture antibody and ab34173 (1/1000) for the detection

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and Azide (ab28947).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody [A180] - BSA and Azide free (ab264434)

    Human breast cancer tissue stained with ab28947 NQO1 antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and Azide (ab28947).

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