Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17829-9] to SHP2 - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free
    See all SHP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17829-9] to SHP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, HEK-293 and NIH/3T whole cell lysates; mouse brain and heart lysates; rat brain lysate. IP: HeLa and NIH/3T3 whole cell lysates.

  • General notes

    ab250905 is the carrier-free version of ab187040.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)
    Western blot - Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)
    All lanes : Anti-SHP2 antibody [EPR17829-9] (ab187040) at 1/5000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : PTPN11 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab187040).

    Lanes 1- 2: Merged signal (red and green). Green - ab187040 observed at 68 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab187040 was shown to react with SHP2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266450 (knockout cell lysate ab257618) was used. Wild-type HEK-293T and PTPN11 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab187040 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)
    Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)

    This data was developed using ab187040, the same antibody clone in a different buffer formulation.

    SHP2 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab187040 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab187040 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input).

    Lane 2: ab187040 IP in HeLa whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187040 in HeLa whole cell lysate (-).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)
    Western blot - Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)
    All lanes : Anti-SHP2 antibody [EPR17829-9] (ab187040) at 1/5000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 5 : Mouse brain tissue lysate
    Lane 6 : Mouse heart tissue lysate
    Lane 7 : Rat brain tissue lysate
    Lane 8 : Rat heart tissue lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa


    Exposure time: 1 minute


    This data was developed using ab187040, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)
    Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)

    This data was developed using ab187040, the same antibody clone in a different buffer formulation.

    SHP2 was immunoprecipitated from 1 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab187040 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab187040 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10µg (Input).

    Lane 2: ab187040 IP in NIH/3T3 whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187040 in NIH/3T3 whole cell lysate (-).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)
    Anti-SHP2 antibody [EPR17829-9] - BSA and Azide free (ab250905)

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