Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (ab251180)
![Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (ab251180)](https://www.abcam.com/ps/products/251/ab251180/Images/ab251180-405707-anti-nat2-at-2-antibody-epr15856-bsa-and-azide-free-western-blot.jpg "Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (ab251180)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15856] to NAT2/AT-2 - BSA and Azide free
- Suitable for: WB, ICC
- Reacts with: Human
Overview
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Product name
Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free
See all NAT2/AT-2 primary antibodies -
Description
Rabbit monoclonal [EPR15856] to NAT2/AT-2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251180 is the carrier-free version of ab194114 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251180 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as NAT2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15856 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (ab251180)Anti-NAT2/AT-2 antibody [EPR15856] (ab194114) at 1/5000 dilution + Human fetal liver tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaThis data was developed using ab194114, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (ab251180)](https://www.abcam.com/ps/products/251/ab251180/Images/ab251180-403340-anti-nat2-at-2-antibody-epr15856-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (ab251180)Anti-NAT2/AT-2 antibody [EPR15856] (ab194114) at 1/1000 dilution + Human colon cancer lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaThis data was developed using ab194114, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunocytochemistry - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (ab251180)](https://www.abcam.com/ps/products/251/ab251180/Images/ab251180-1-anti-nat2-at-2-antibody-epr15856-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (ab251180)This data was developed using ab194114, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling NAT2/AT-2 with ab194114 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue). -
![Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (ab251180)](https://www.abcam.com/ps/products/251/ab251180/Images/ab251180-4-benefits-of-recombinant-antibodies.png)
Anti-NAT2/AT-2 antibody [EPR15856] - BSA and Azide free (ab251180)